Download e-book for kindle: Alcohol and abnormal protein biosynthesis; biochemical and by Marcus Adolphus Rothschild; Murray Oratz; Sidney S Schreiber

By Marcus Adolphus Rothschild; Murray Oratz; Sidney S Schreiber

ISBN-10: 0080177085

ISBN-13: 9780080177083

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Next, the mechanism of protein synthesis on ribosomes in the cell cytoplasm will be examined in order to identify sites of control by amino acid supply. This will be followed by a section on the responses of RNA and nucleotide metabolism to amino acid availability. Finally, an attempt will be made to integrate these acute responses to amino acid supply into a single picture. II. RESPONSE OF HEPATIC PROTEIN SYNTHESIS TO AMINO ACID SUPPLY Following protein-containing meals, the liver is subjected to considerable fluctuations in amino acid supply.

Table 3 shows that absence of tryptophan from the amino acid mixture led to the greatest reduction in charging of the corresponding tRNA, followed by valine, isoleucine, and 48 H. N. MUNRO, CHRISTINE HUBERT, AND B. SUREN BALIGA Table 3 Charging levels of hepatic tRNA after tube-feeding complete or deficient mixtures of amino acids to rats. Amino Acid Omitted tRNA Charging (%) Complete Mixture Deficient Mixture Difference Tryptophan Valine Isoleucine 77 92 80 29 54 51 - 48 - 38 - 29 Phenylalanine Leucine Lysine Arginine 81 86 89 94 66 85 86 86 - 15 -1 -3 -8 Data from Table IV and footnote p.

J_ I- - w z (/) 0 � I- 50 0 1------�-"--�_J__---��--'�-I L7 LS L9 LIO Lil LIZ D I D2 D3 D4 D5 TI M E D6 OF D7 D8 DAY D9 DIO Dll 012 LI L2 L3 L4 L5 Fig. 3 Hepatic polysome profiles and tyrosine transaminase activity in groups of 6 rats fed Purina Chow, killed at indicated times. Vertical bars = standard errors of the mean, and hatching along the abscissa indicates the daily dark period [ 1 6]. ) 38 H. N. MUNRO, CHRISTINE HUBERT, AND B. SUREN BALIGA of amino acids away from liver and into muscle when a carbohydrate meal is consumed [17] .

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Alcohol and abnormal protein biosynthesis; biochemical and clinical by Marcus Adolphus Rothschild; Murray Oratz; Sidney S Schreiber


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