Read e-book online Affinity Chromatography PDF

By Jaroslava Turková (Eds.)

ISBN-10: 0444416056

ISBN-13: 9780444416056

Show description

Read Online or Download Affinity Chromatography PDF

Best analytic books

Download e-book for kindle: Advances in Chromatography: Volume 48 by Eli Grushka, Nelu Grinberg

For greater than 4 many years, scientists and researchers have depended on the Advances in Chromatography sequence for the main updated info on quite a lot of advancements in chromatographic tools and functions. With contributions from an array of foreign specialists, the newest quantity captures new advancements during this very important box that yields nice probabilities in a few purposes.

Tullio Valent's Boundary Value Problems of Finite Elasticity: Local Theorems PDF

During this publication I current, in a scientific shape, a few neighborhood theorems on lifestyles, strong point, and analytic dependence at the load, which i've got lately got for a few sorts of boundary worth difficulties of finite elasticity. really, those effects problem an n-dimensional (n ~ 1) formal generalization of third-dimensional elasticity.

Additional resources for Affinity Chromatography

Sample text

From the agreement of the KL and KI values it can soluble antilysine K I = 8 9 be concluded that the formation of the complex between chymotrypsin and antilysine is not affected by its covalent bond with Spheron. The dissociation constants for the complex of chymotrypsin and antilysine, either in solution or bound to a solid matrix, are in good agreement. 8 - 1 Od6 M). 2-10-5 M, which agrees closely with the published value of 3 lo-’ M (Walsh and Wilcox). 2 DETERMINATION OF DISSOCIATION CONSTANTS BY FRONTAL ANALYSIS The derivation of quantitative affinity chromatography by frontal analysis (Kasai and Ishii) is based on the assumption that if the total length of the agarose column is 1 and the total amount of the immobilized ligand in the adsorbent is Lt, then Lo = Lt/l, where [Lo] is the density of affinity ligands per unit length of the agarose column.

Limited the analysis t o short oligomers and were * For list of symbols see p. 33. THEORY GF AFFINITY CHROMATOGRAPHY 28 able to assume an all-or-none binding of the oligomer on to polyuridylic acid. 32) x s N F p N { [ u p ( 2-p)/(l -p)'] + 1 ) + N - 1 From eqn. 33) ,'. i' . ,','. ' .. -.. I . 0 1Qo [FREE O L l G O M E R l (MOLES/LITER 10'1 Fig. 8. 033 M phosphate buffer, 1 M NaCl, 1 mM MgCl,. 9 lW4 M. Broken line, theoretical curve. The ordinate indicates the helical content of polyuridylic acid in 1 : 2 stoichiometry of adenylic acid : uridylic acid.

Acta, 354 (1974) 121-129. , Biochem. Biophys. Res. ,38 (1970) 875-881. J. , Eur. J. , 41 (1974a) 341-345. J. , Eur. J. Biochem.. 42 (1974b) 1-6. H. ,Biopolymers, 1 (1963) 133-143. , Bailon, P. , Advan. Exp. Med. , 42 (1974) 33-42. , Tanabe, S. , Biopolymers, 14 (1975) 33-49. , 34 (1974) 13-30. Porath, J. , in H. L. 95-178. H. , Chromarographia, 4 (1971) 578-581. , Cuatrecasas, P. , J. Bwl. , 246 (1971) 196-200. , Coupek, J. ,Eiochim. Biophys, Acta, 427 (1976) 586-593. 35 Chapter 4 Application of affinity chromatography to the quantitative evaluation of specific complexes Association processes undoubtedly play one of the most important roles in biochemical processes.

Download PDF sample

Affinity Chromatography by Jaroslava Turková (Eds.)


by John
4.1

Rated 4.30 of 5 – based on 12 votes