Download PDF by A.H. Rose and J. Gareth Morris (Eds.): Advances in Microbial Physiology, Vol. 21

By A.H. Rose and J. Gareth Morris (Eds.)

ISBN-10: 0120277212

ISBN-13: 9780120277216

This quantity in a research-level sequence covers varied features of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial telephone partitions. it truly is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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Additional resources for Advances in Microbial Physiology, Vol. 21

Sample text

2 . Transcription in Isolated Mitochondria A single paper has appeared to date dealing with in uitro transcription in isolated mitochondria from P . polyqbhalum (Grant and Poulter, 1973). Here R N A synthesis is inhibited both by actinomycin D and by rifampicin (50% inhibition by both drugs is exhibited at around 5 pg/ml). The half-life of R N A synthesized in uitro is extremely short, namely around 2 to 3 min. This suggests, unless the rapid turnover is an experimental artefact, that the molecuks made in uitro are not of ribosomal RNA, since ribosomal R N A would be expected to be much more stable.

The activity of this factor (measured as stimulation of RNA polymerase I1 activity in vitro) gradually decreases during starvation to undetectable levels. From the evidence presently available it is difficult to judge if its activity, and hence its disappearance, is of consequence for one o r othei- o r both of the two RNA po lymerases. Taking into account all the observations outlined above, a picture of the regulation of transcription during differentiation emerges wherein the overall concentration of potentially active RNA polymerases I and I1 remains constant throughout starvation.

In P . polycephalum some of the biochemical events of the mitotic cycle have been studied in considerable detail. , 1965; Braun and Wili, 1969). , 1974a; Fouquet and Sauer, 1975). , 1972). The second minor DNA fraction, mitochondria1 DNA, replicates throughout the intermitotic period, as was found in several other eukaryotic cell types (Sonenshein and Holt, 1968; Braun and Evans, 1969). 1. Dtflerential Transcription in the Mitotic Cycle How does transcription relate to the mitotic cycle? A decrease of RNA synthesis during mitosis was already indicated by Nygaard et al.

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Advances in Microbial Physiology, Vol. 21 by A.H. Rose and J. Gareth Morris (Eds.)


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