Download PDF by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.): Advanced Flow Cytometry: Applications in Biological Research

By L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)

ISBN-10: 9048163684

ISBN-13: 9789048163687

ISBN-10: 9401706239

ISBN-13: 9789401706230

Flow cytometry has quickly developed right into a procedure for quick research of DNA content material, mobile marker expression and digital sorting of cells of curiosity for additional investigations. move cytometers are being commonly used for tracking of mobile DNA content material, phenotype expression, drug delivery, calcium flux, proliferation and apoptosis. Phenotypic research of marker expression in leukemic cells has turn into an enormous software for diagnostic and healing tracking of sufferers. contemporary reviews have explored using stream cytometry for tracking hormone receptor expression in human good tumors and for experiences in human genomics. Contributions within the present quantity are in line with shows made on the First Indo-US workshop on stream Cytometry within which specialists from united states, united kingdom and India mentioned functions of move cytometry in organic and scientific examine. This publication can be of curiosity to put up graduates and researchers within the fields of pathology, cytology, mobile biology and molecular biology.

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Franklin Lakes, NJ, USA. , St. , St. , St. Louis, MO, USA Anti-fade solution: Slow Fade (S-2828): Molecular Probes, Eugene, OR References I. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F (1996). Cytometry of cyclin proteins. Cytometry 25: 1-13. 2. Kakino S, Sasaki K, Kurose A, Ito H (1996). Intracellular localization of cyclin B 1 during the cell cycle in glioma cells. Cytometry 24: 49-54. 3. Kallioniemi O-P, Wagner U, Kononen J, Sauter G (2001). Tissue micro array technology for highthroughput molecular profiling of cancer.

In: Methods in Cell Biology, Vol. 33, pp. 377-383 (1990) . • L. S. Cram, F. A. Ray, and M. F. Bartholdi, Univariate Analysis of Metaphase Chromosomes Using the Hypotonic Potassium ChloridePropidium Iodide Protocol. In: Methods in Cell Biology, Vol. 33, pp. 369-377 (1990). Acknowledgements Collaborators at the National Flow Cytometry Resource, Los Alamos National Laboratory, who have made outstanding contributions to our development of flow cytogenetics include James Jett, Carolyn Bell, Joe Fawcett, John Martin, and Larry Deaven.

O~-L~~L-~~~~~~~~~~~~~~ o 123 E 4 5 678 9 1011121314151617181920212223 GI z s Figure 3. Continued. hour 46 25 . Measure cyclin (cyclin A or B 1) and DNA contents simultaneously with LSC as described below. 5. LSC measurement of cyclins and nuclear DNA Amounts of cellular cyclins and nuclear DNA are measured concomitantly with a laser scanning cytometer (LSC2, CompuCyte, Cambridge, MA) for all spots at once. Scanning is limited to the area containing spots, and inter-spot areas are excluded from scanning by computer software.

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Advanced Flow Cytometry: Applications in Biological Research by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)

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